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ej human bladder carcinoma cell line  (ATCC)


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    ATCC ej human bladder carcinoma cell line
    Inhibition of the proliferation of EJ <t>bladder</t> cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). <t>Cell</t> viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, <t>human</t> urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
    Ej Human Bladder Carcinoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ej+human+bladder+carcinoma+cell+line/J82%3B+Bladder+Carcinoma%3B+Human/pmc05987858-52-1-10
    Average 94 stars, based on 120 article reviews
    ej human bladder carcinoma cell line - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Inhibitory effect of Au@Pt-NSs on proliferation, migration, and invasion of EJ bladder carcinoma cells: involvement of cell cycle regulators, signaling pathways, and transcription factor-mediated MMP-9 expression"

    Article Title: Inhibitory effect of Au@Pt-NSs on proliferation, migration, and invasion of EJ bladder carcinoma cells: involvement of cell cycle regulators, signaling pathways, and transcription factor-mediated MMP-9 expression

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S158463

    Inhibition of the proliferation of EJ bladder cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). Cell viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, human urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
    Figure Legend Snippet: Inhibition of the proliferation of EJ bladder cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). Cell viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, human urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

    Techniques Used: Inhibition, Incubation, MTT Assay, Staining, Control, Negative Control

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    Modification:

    Article Title: Inhibitory effect of Au@Pt-NSs on proliferation, migration, and invasion of EJ bladder carcinoma cells: involvement of cell cycle regulators, signaling pathways, and transcription factor-mediated MMP-9 expression
    Article Snippet: The nuclear extract kit and the electrophoretic mobility shift assay (EMSA) gel shift kit were obtained from Panomics (Fremont, CA, USA). .. The EJ human bladder carcinoma cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a 5% CO 2 humidified incubator. .. Normal human urothelial cells (HUCs) were obtained from ScienCell Research Laboratories (Carlsbad, CA, USA) and grown in the medium specific for HUCs with supplements according to the manufacturer’s protocol.



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    Inhibition of the proliferation of EJ <t>bladder</t> cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). <t>Cell</t> viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, <t>human</t> urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
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    Inhibition of the proliferation of EJ <t>bladder</t> cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). <t>Cell</t> viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, <t>human</t> urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
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    Inhibition of the proliferation of EJ <t>bladder</t> cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). <t>Cell</t> viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, <t>human</t> urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
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    Inhibition of the proliferation of EJ bladder cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). Cell viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, human urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

    Journal: International Journal of Nanomedicine

    Article Title: Inhibitory effect of Au@Pt-NSs on proliferation, migration, and invasion of EJ bladder carcinoma cells: involvement of cell cycle regulators, signaling pathways, and transcription factor-mediated MMP-9 expression

    doi: 10.2147/IJN.S158463

    Figure Lengend Snippet: Inhibition of the proliferation of EJ bladder cancer cells by heterometallic Au@Pt-NSs. Notes: Both EJ cells and HUCs were incubated for 24 h with different concentrations of Au@Pt-NSs (0, 0.1, 0.3, and 0.5 μM). Cell viability was measured using the MTT assay in EJ cells ( A ) and HUCs ( D ). Viable cells were measured using trypan blue staining for EJ cells ( B ) and HUCs ( E ). Morphological changes were observed after treatment with Au@Pt-NSs at different concentrations (0, 0.1, 0.3, and 0.5 μM) for EJ cells ( C ) and HUCs ( F ). For the bar graphs, values are presented as mean ± SD of three independent experiments; * P < 0.05, compared with the control group. Abbreviations: Au@Pt-NSs, gold@platinum nanoseeds; Con, negative control; HUCs, human urothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

    Article Snippet: The EJ human bladder carcinoma cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a 5% CO 2 humidified incubator.

    Techniques: Inhibition, Incubation, MTT Assay, Staining, Control, Negative Control